yisX

168

binds to DNA lesions, may be involved in DNA damage repair

locus: BSU_10890

Molecular weight: 23.58 kDa

pI: 5.05

Protein length: 212 aa

Gene length: 639 bp

function: unknown

product: unknown

essential: no

ec: null

synonyms: yisX

List of homologs in different organisms, belongs to COG1357 (Galperin et al., 2021)

Gene

Coordinates: 1,168,199  1,168,837

The protein

Catalyzed reaction/ biological activity

• binds to 8-oxoG and RNA base DNA lesions [pubmed|37250769]

[wiki|Domains]

• 3 Pentapeptide repeat domains (aa 63-102, aa 103-142, aa 143-182) (according to UniProt)

Structure

• [PDB|6FLS] (from Clostridium botulinum, corresponds to aa 28 ... 235, 24% identity) [pubmed|30539762]
• [AF|O06733]

Expression and Regulation

Operons

• genes: [gene|5237C86931876179C9CE67A9B04A0A62ADA7C25C|yisX]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B193 (yisX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1192 NBRP B. subtilis, Japan]
• BKE10890 ([gene|5237C86931876179C9CE67A9B04A0A62ADA7C25C|yisX]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10890 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGATGACATCCTTTGC,  downstream forward: _UP4_GGAGCTGTCATCGTATAACC
• BKK10890 ([gene|5237C86931876179C9CE67A9B04A0A62ADA7C25C|yisX]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10890 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGATGACATCCTTTGC,  downstream forward: _UP4_GGAGCTGTCATCGTATAACC

References

Research papers

• 30539762,37250769