yisI

168

Spo0A-P phosphatase, control of the phosphorelay

Locus: BSU_10730

Molecular weight: 6.54 kDa

Isoelectric point: 5.12

Protein length: 56 aa

Gene length: 171 bp

Function: control of sporulation initiation

Product: Spo0A-P phosphatase

Essential: no

Synonyms: yisI

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5804 (Galperin et al., 2021)

Gene

Coordinates: 1,150,850  1,151,020

The protein

Protein family

• spo0E family (with ynzd and Spo0E, according to UniProt)

Structure

• 2C0S (PDB) (from B. anthracis, corresponds to aa 1 ... 41 of YisI, 44% identity) PubMed
• O06722 (AlphaFold)

Expression and Regulation

Operons

• Genes: yisI

  Description: PubMed

  Regulatory mechanism

  • PchR: activation, in pchR regulon

• Genes: yisJ-yisI

  Description: PubMed

  Regulation

  • induced during sporulation PubMed
  • strongly expressed during oligotrophic growth PubMed

Biological materials

Mutant

• BKE10730 (yisI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACGCTATCTCCAATTC,  downstream forward: _UP4_TAAATCATTTTCTATAACAA
• BKK10730 (yisI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACGCTATCTCCAATTC,  downstream forward: _UP4_TAAATCATTTTCTATAACAA

References

Loading