ntdC

168

NAD-dependent glucose-6-phosphate dehydrogenase

Locus: BSU_10530

Molecular weight: 39.14 kDa

Isoelectric point: 6.23

Protein length: 350 aa

Gene length: 1053 bp

Function: synthesis of the antibiotic kanosamine

Product: NAD-dependent glucose-6-phosphate dehydrogenase

Essential: no

Synonyms: ntdC, yhjJ

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0673 (Galperin et al., 2021)

Gene

Coordinates: 1,126,400  1,127,452

The protein

Catalyzed reaction/ biological activity

• glucose-6-phosphate + NAD - 3-oxo-d-glucose-6-phosphate + NADH(2) PubMed

Protein family

• Gfo/Idh/MocA family (according to UniProt)

Cofactors

• NAD⁺ PubMed

Structure

• 3EUW (PDB) (from Corynebacterium glutamicum, 21% identity)
• O07564 (AlphaFold)

Paralogous protein(s)

• YteT, IolW, IolU, IolG, YrbE, IolX, YfiI

Expression and Regulation

Operons

• Genes: ntdA-ntdB-ntdC-glcP

  Description: PubMed

  Regulation

  • induced by 3,3'-neotrehalosadiamine and kanosamine (NtdR) PubMed

  Regulatory mechanism

  • NtdR: activation , in ntdR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B286 (yhjJ::erm), available at the NBRP B. subtilis, Japan
• BKE10530 (ntdC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTATTTCCTCCTCAT,  downstream forward: _UP4_TAGTATTTCAAAGAGAGAAG
• BKK10530 (ntdC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTATTTCCTCCTCAT,  downstream forward: _UP4_TAGTATTTCAAAGAGAGAAG

References

Reviews

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Original Publications

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