yhxD

168

general stress protein, similar to alcohol dehydrogenase

Locus: BSU_10430

Molecular weight: 31.76 kDa

Isoelectric point: 4.54

Protein length: 299 aa

Gene length: 900 bp

Function: survival of salt and ethanol stresses

Product: unknown

Essential: no

Synonyms: yhxD

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1028 (Galperin et al., 2021)

Gene

Coordinates: 1,117,734  1,118,633

The protein

Protein family

• Short-chain dehydrogenases/reductases (SDR) family (according to UniProt)

Structure

• 3R3S (PDB) (from Salmonella Enterica, 66% identity)
• P40398 (AlphaFold)

Paralogous protein(s)

• YxbG, BacC, FabG
• YcdF, (31,1%)
• Gdh, (33,5%)
• YhdF, (47%)
• YdaD, (48,8%)
• YkvO, (34,5%)
• YhxC, (45,2%),

Expression and Regulation

Operons

• Genes: yhxD-as-comK-yhzC

  Description

  • PubMed

  Regulation

  • induced under stress conditions (SigB), requires read through at the terminator downstream of yhxD PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

  Additional information

  • the transcript is cleaved between the as-comK and yhzC segments PubMed

• Genes: yhxD

  Description: PubMed

  Regulation

  • induced by stress (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• MGNA-A719 (yhxD::erm), available at the NBRP B. subtilis, Japan
• BKE10430 (yhxD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAGCAACGCTCCTTTTC,  downstream forward: _UP4_TAGCTTTATGAAAGGAGCTG
• BKK10430 (yhxD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAGCAACGCTCCTTTTC,  downstream forward: _UP4_TAGCTTTATGAAAGGAGCTG

References

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