yhfW

168

similar to Rieske 2Fe-2S iron-sulfur protein

Locus: BSU_10390

Molecular weight: 56.94 kDa

Isoelectric point: 5.66

Protein length: 509 aa

Gene length: 1530 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yhfW

Genomic Context

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List of homologs in different organisms, belongs to COG0723 (Galperin et al., 2021)

Gene

Coordinates: 1,114,057  1,115,586

Phenotypes of a mutant

• reduced transfomation efficiency PubMed
• reduced amounts of citric acid cycle intermediates PubMed

The protein

Domains

• Rieske domain (aa 423-509) (according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 4P9S (PDB) (dimethylglycine dehydrogenase from Rattus norvegicus, corresponds to aa 22 ... 396, 25% identity) PubMed
• O07622 (AlphaFold)

Expression and Regulation

Operons

• Genes: yhfW

  Description: PubMed

  Regulation

  • expressed during sporulation in the forespore (SigF) PubMed

  Sigma factors

  • SigF: sigma factor, PubMed, in sigF regulon

Biological materials

Mutant

• MGNA-A716 (yhfW::erm), available at the NBRP B. subtilis, Japan
• BKE10390 (yhfW::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGCCGTTATACCTCCAAT,  downstream forward: _UP4_TAAACCGTTCATTTTAATGA
• BKK10390 (yhfW::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGCCGTTATACCTCCAAT,  downstream forward: _UP4_TAAACCGTTCATTTTAATGA

References

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