lcfB

168

long-chain fatty-acid-CoA ligase, involved in surfactin production

Locus: BSU_10270

Molecular weight: 56.41 kDa

Isoelectric point: 5.3

Protein length: 513 aa

Gene length: 1542 bp

Function: fatty acid degradation

Product: long-chain fatty-acid-CoA ligase

Essential: no

E.C.: 6.2.1.3

Synonyms: lcfB, yhfL

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0318 (Galperin et al., 2021)

Gene

Coordinates: 1,100,980  1,102,521

The protein

Catalyzed reaction/ biological activity

• long-chain fatty acid + ATP + CoA --> long-chain fatty acyl-CoA + AMP + diphosphate (according to UniProt)
• activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed

Protein family

• ATP-dependent AMP-binding enzyme family (according to UniProt)

Structure

• 3R44 (PDB) (Very-long-chain fatty acyl-CoA synthetase from Mycobacterium tuberculosis, 35% identity) PubMed
• O07610 (AlphaFold)

Paralogous protein(s)

• YhfT, YngI, LcfA

Expression and Regulation

Operons

• Genes: lcfB

  Description: PubMed

  Regulation

  • induced in the presence of long-chain fatty acids (FadR) PubMed
  • down-regulated in response to RoxS overexpression PubMed

  Regulatory mechanism

  • FadR: repression, PubMed, in fadR regulon
  • CcpA: repression, in ccpA regulon
  • RoxS: sRNA, PubMed, in roxS regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B277 (yhfL::erm), available at the NBRP B. subtilis, Japan
• available in Mohamed Marahiel's lab PubMed
• BKE10270 (lcfB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTCCCACCCCTTAAGT,  downstream forward: _UP4_TAAAATGAAAAAGCGAAGCG
• BKK10270 (lcfB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTCCCACCCCTTAAGT,  downstream forward: _UP4_TAAAATGAAAAAGCGAAGCG

References

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