yhcR

168

extracellular non-specific nuclease, degrades DNA in the biofilm to release phosphate ions

Locus: BSU_09190

Molecular weight: 132.47 kDa

Isoelectric point: 4.69

Protein length: 1217 aa

Gene length: 3654 bp

Function: utilization of nucleic acids in biofilms

Product: extracellular non-specific nuclease, DNase

Essential: no

E.C.: null

Synonyms: yhcR

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4085 (Galperin et al., 2021)

Gene

Coordinates: 991,348 → 995,001

The protein

Catalyzed reaction/ biological activity

• degradation of extracellular DNA in biofilms to obtain phosphate ions PubMed

Protein family

• 5'-nucleotidase family (with YfkN, according to UniProt)

Domains

• TNase-like domain (aa 376-517) (according to UniProt)

Cofactors

• Ca(2+) PubMed

Structure

• 2Z1A (PDB) (from Thermus thermophilus, corresponds to aa 590 ... 1094, 29% identity)
• P54602 (AlphaFold)

Paralogous protein(s)

• YfkN

Localization

• extracellular (signal peptide) PubMed, attached to the cell wall by sorties YhcR PubMed

Expression and Regulation

Operons

• Genes: yhcR-yhcS

  Description: PubMed

Biological materials

Mutant

• MGNA-A665 (yhcR::erm), available at the NBRP B. subtilis, Japan
• BKE09190 (ΔyhcR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATAATTCCTTTCAGGCT,  downstream forward: _UP4_AGAAAAAGGAGCGCCTCCAG
• BKK09190 (ΔyhcR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATAATTCCTTTCAGGCT,  downstream forward: _UP4_AGAAAAAGGAGCGCCTCCAG

Labs working on this gene/protein

• David Bechhofer, Mount Sinai School, New York, USA Homepage

References

Reviews

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Original Publications

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