queG

168

epoxyqueuosine reductase

Locus: BSU_08910

Molecular weight: 48.37 kDa

Isoelectric point: 7.35

Protein length: 386 aa

Gene length: 1161 bp

Function: synthesis of queuosine, tRNA modification

Product: epoxyqueuosine reductase

Essential: no

Synonyms: queG, ygaP, yhbA

Genomic Context

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List of homologs in different organisms, belongs to COG1600 (Galperin et al., 2021)

Gene

Coordinates: 967,935  969,095

The protein

Catalyzed reaction/ biological activity

• conversion of epoxyqueuosine to queuosine PubMed
• AH₂ + epoxyqueuosine³⁴ in tRNA = A + H₂O + queuosine³⁴ in tRNA (according to UniProt)

Protein family

• QueG family (single member, according to UniProt)

Domains

• 4Fe-4S ferredoxin-type domain (aa 178-208) (according to UniProt)

Cofactors

• cobalamin
• Fe-S cluster PubMed

Structure

• 5D08 (PDB) PubMed
• P97030 (AlphaFold)

Expression and Regulation

Operons

• Genes: queG

  Description: PubMed

Biological materials

Mutant

• MGNA-A241 (yhbA::erm), available at the NBRP B. subtilis, Japan
• BKE08910 (queG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTTGCACAGCCTCCT,  downstream forward: _UP4_TGACATACGTATAGAGCAGA
• BKK08910 (queG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTTGCACAGCCTCCT,  downstream forward: _UP4_TGACATACGTATAGAGCAGA

References

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