padR

168

repressor of yveF-yveG-padC, induction occurs by binding of phenolic acids,stress response

Locus: BSU_08340

Molecular weight: 21.08 kDa

Isoelectric point: 7.23

Protein length: 182 aa

Gene length: 549 bp

Function: regulation of the phenolic acid stress response

Product: transcriptional repressor

Essential: no

Synonyms: padR, yfiO

Genomic Context

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List of homologs in different organisms, belongs to COG1695 (Galperin et al., 2021)

Gene

Coordinates: 909,198  909,746

The protein

Domains

• N-terminal winged helix-turn-helix (wHTH) domain (NTD) PubMed
• C-terminal homodimerization domain (CTD) PubMed

Structure

• 5X12 (PDB) (PadR) PubMed
• 5X11 (PDB) (complex with dsDNA) PubMed
• 5X13 (PDB) (complex with p-coumaric acid) PubMed
• P94443 (AlphaFold)

Effectors of protein activity

• phenolic acid acts as molecular inducer and releases PadR from the'' yveF-yveG-padC'' promoter PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: padR

  Description: PubMed

  Regulation

  • weak constitutive expression

Biological materials

Mutant

• MGNA-C303 (yfiO::erm), available at the NBRP B. subtilis, Japan
• BKE08340 (padR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTATCCTCCGTGTCTG,  downstream forward: _UP4_TAACCGCAGTTCAGGCTGCA
• BKK08340 (padR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTATCCTCCGTGTCTG,  downstream forward: _UP4_TAACCGCAGTTCAGGCTGCA

References

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