lnrN

168

similar to ABC transporter (membrane protein), involved in resistance to linearmycin

Locus: BSU_08330

Molecular weight: 41.92 kDa

Isoelectric point: 9.29

Protein length: 385 aa

Gene length: 1158 bp

Function: resistance to linearmycin

Product: ABC transporter (membrane protein)

Essential: no

Synonyms: yfiN

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0842 (Galperin et al., 2021)

Gene

Coordinates: 907,968  909,125

Phenotypes of a mutant

• overexpression of lnrM and lnrN stimulates biofilm formation PubMed

The protein

Protein family

• ABC-2 integral membrane protein family (according to UniProt)

Domains

• ABC transmembrane type-2 domain (aa 163-382) (according to UniProt)

Structure

• P94442 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: lnrL-lnrM-lnrN

  Description: PubMed

  Regulation

  • expression during spore germination is increased under conditions of osmotic stress PubMed
  • induced in the presence of linearmycin and other polyenes (LnrK) PubMed

  Regulatory mechanism

  • LnrK: activation, PubMed, in lnrK regulon

  Sigma factors

  • SigF: sigma factor, in sigF regulon
  • SigG: sigma factor, in sigG regulon

Biological materials

Mutant

• MGNA-C355 (yfiN::erm), available at the NBRP B. subtilis, Japan
• BKE08330 (lnrN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATTTTTTTCACCTTTTGTA,  downstream forward: _UP4_TAAAAAACATCTGCCGTTTA
• BKK08330 (lnrN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATTTTTTTCACCTTTTGTA,  downstream forward: _UP4_TAAAAAACATCTGCCGTTTA

References

Loading