yfkJ

168

general stress protein, protein tyrosine phosphatase, required for protection against paraquat stress

Locus: BSU_07880

Molecular weight: 17.13 kDa

Isoelectric point: 4.56

Protein length: 156 aa

Gene length: 471 bp

Function: survival of stress conditions

Product: protein tyrosine phosphatase

Essential: no

E.C.: 3.1.3.48

Synonyms: yfkJ

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0394 (Galperin et al., 2021)

Gene

Coordinates: 862,004  862,474

The protein

Catalyzed reaction/ biological activity

• H₂O + O-phospho-L-tyrosyl-[protein] --> L-tyrosyl-[protein] + phosphate (according to UniProt)

Protein family

• low molecular weight phosphotyrosine protein phosphatase family (with ArsC and YwlE, according to UniProt)

Structure

• 4ETM (PDB)
• O35016 (AlphaFold)

Paralogous protein(s)

• YwlE

Expression and Regulation

Operons

• Genes: yfkJ-yfkI-yfkH

  Description: PubMed

  Regulation

  • induced by stress (SigB) PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• MGNA-C266 (yfkJ::erm), available at the NBRP B. subtilis, Japan
• BKE07880 (yfkJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGTGCAACCTCCCTA,  downstream forward: _UP4_TTGTGAGTGAAAGGAGAATT
• BKK07880 (yfkJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGTGCAACCTCCCTA,  downstream forward: _UP4_TTGTGAGTGAAAGGAGAATT

References

Loading