yetK

168

unknown

Locus: BSU_07210

Molecular weight: 35.60 kDa

Isoelectric point: 9.53

Protein length: 330 aa

Gene length: 993 bp

Function: unknown

Product: unknown

Essential: no

E.C.: null

Synonyms: yetK

Genomic Context

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List of homologs in different organisms, belongs to COG0697 (Galperin et al., 2021)

Gene

Coordinates: 788,636  789,628

The protein

Protein family

• EamA transporter family (according to UniProt)

Domains

• 2 EamA domains (aa 38-163, 187-314) (according to UniProt)

Structure

• O31540 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: yetJ-yetK

  Description: PubMed

• Genes: yezD-yetJ-yetK

  Description: PubMed

Biological materials

Mutant

• MGNA-B462 (yetK::erm), available at the NBRP B. subtilis, Japan
• BKE07210 (yetK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAATCATCACTGCTCAA,  downstream forward: _UP4_TAGCCTGTCCGGATCGGACG
• BKK07210 (yetK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAATCATCACTGCTCAA,  downstream forward: _UP4_TAGCCTGTCCGGATCGGACG

Expression system

• GP4400 (trpC2 xkdE::P_xyl-yetK-ermC), available in Jörg Stülke's lab

Original Publications

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