yetK

168

unknown

Locus: BSU_07210

Molecular weight: 35.60 kDa

Isoelectric point: 9.53

Protein length: 330 aa

Gene length: 993 bp

Function: unknown

Product: unknown

Essential: no

E.C.: null

Synonyms: yetK

Genomic Context

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Categories containing this gene/protein

Groups of genes, Membrane proteins

Groups of genes, Proteins of unknown function

List of homologs in different organisms, belongs to COG0697 (Galperin et al., 2021)

Gene

Coordinates: 788,636  789,628

The protein

Protein family

EamA transporter family (according to UniProt)

Domains

2 EamA domains (aa 38-163, 187-314) (according to UniProt)

Structure

O31540 (AlphaFold)

Localization

cell membrane (according to UniProt)

Expression and Regulation

Operons

Genes: yetJ-yetK

Description: PubMed

Genes: yezD-yetJ-yetK

Description: PubMed

Biological materials

Mutant

MGNA-B462 (yetK::erm), available at the NBRP B. subtilis, Japan
BKE07210 (yetK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAATCATCACTGCTCAA,  downstream forward: _UP4_TAGCCTGTCCGGATCGGACG
BKK07210 (yetK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAATCATCACTGCTCAA,  downstream forward: _UP4_TAGCCTGTCCGGATCGGACG

Expression system

GP4400 (trpC2 xkdE::P_xyl-yetK-ermC), available in Jörg Stülke's lab

Original Publications

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