yetF

168

required for wet heat resistance of spores

locus: BSU_07140

Molecular weight: 26.16 kDa

pI: 7.09

Protein length: 231 aa

Gene length: 696 bp

function: stabilization of spore inner membrane proteins against wet heat

product: inner spore membrane protein

essential: no

synonyms: yetF

List of homologs in different organisms, belongs to COG2323 (Galperin et al., 2021)

Gene

Coordinates: 784,381 → 785,076

Phenotypes of a mutant

• reduced wet heat resistance [pubmed|37113233]
• decreased spore resistance to heat and chemicals [pubmed|40938645]
• deficient in spore germination in response to AGFK [pubmed|37113233]

The protein

Protein family

• [wiki|UPF0702 family] (according to UniProt)

Structure

• [PDB|3C6F]
• [AF|O31533]

Paralogous protein(s)

• [protein|B37938C639FC87DAA72AA82998DA8DF25E63CF37|ydfS]

[wiki|Localization]

• inner spore membrane [pubmed|37113233]

Expression and Regulation

Operons

• genes: [gene|18B09A645AC8E13A1AD3B73A0C828E5A0F5D42BB|lplD]-[gene|EBD0F966FD7B3662814D530064BD307561A5CB15|yetF]

  description: [pubmed|22383849]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|26577401], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]

Biological materials

Mutant

• MGNA-B457 (yetF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1456 NBRP B. subtilis, Japan]
• BKE07140 (Δ[gene|EBD0F966FD7B3662814D530064BD307561A5CB15|yetF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07140 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGATAGCACCTACTT,  downstream forward: _UP4_TGACTCTTAGAAAAAAGCTT
• BKK07140 (Δ[gene|EBD0F966FD7B3662814D530064BD307561A5CB15|yetF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07140 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGATAGCACCTACTT,  downstream forward: _UP4_TGACTCTTAGAAAAAAGCTT

References

• 26577401,35638784,37113233,40938645