rhgK

168

two-component sensor kinase

Locus: BSU_06950

Molecular weight: 66.17 kDa

Isoelectric point: 7.28

Protein length: 577 aa

Gene length: 1734 bp

Function: unknown

Product: two-component sensor kinase

Essential: no

E.C.: null

Synonyms: yesM

Genomic Context

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List of homologs in different organisms, belongs to COG2972 (Galperin et al., 2021)

Gene

Coordinates: 758,719  760,452

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of RhgL
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

Domains

• two transmembrane segments
• HAMP domain (aa 312-368) (according to UniProt)
• Histidine kinase domain (aa 365-574) (according to UniProt)

Structure

• O31516 (AlphaFold)

Modification

• autophosphorylation on a His residue

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: yesL-rhgK-rhgL

  Description: PubMed

  Regulation

  • induced by pectin PubMed
  • repressed by glucose (CcpA) PubMed

  Regulatory mechanism

  • CcpA: repression, in ccpA regulon

Biological materials

Mutant

• MGNA-A945 (yesM::erm), available at the NBRP B. subtilis, Japan
• BKE06950 (rhgK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTACCAGCCAGCAACTCTTT,  downstream forward: _UP4_CCGTGCCGAAATGAGGTGGT
• BKK06950 (rhgK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTACCAGCCAGCAACTCTTT,  downstream forward: _UP4_CCGTGCCGAAATGAGGTGGT

References

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