pcrB

168

heptaprenylglyceryl phosphate synthase

Locus: BSU_06600

Molecular weight: 24.94 kDa

Isoelectric point: 4.2

Protein length: 228 aa

Gene length: 687 bp

Function: unknown

Product: heptaprenylglyceryl phosphate synthase

Essential: no

Synonyms: pcrB, yerE

Genomic Context

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Categories containing this gene/protein

Metabolism, Lipid metabolism, Lipid metabolism/ other

List of homologs in different organisms, belongs to COG1646 (Galperin et al., 2021)

This gene is a member of the following regulons

LexA regulon

Gene

Coordinates: 718,622  719,308

The protein

Catalyzed reaction/ biological activity

production of heptaprenylglyceryl phosphate from heptaprenyl diphosphate and glycerol-1-phosphate PubMed
all-trans-heptaprenyl diphosphate + sn-glycerol 1-phosphate --> 3-heptaprenyl-sn-glycero-1-phosphate + diphosphate (according to UniProt)

Protein family

GGGP/HepGP synthase family (single member, according to UniProt)

Structure

1VIZ (PDB) PubMed
O34790 (AlphaFold)

Expression and Regulation

Operons

Genes: pcrB-pcrA-ligA-yerH

Description: PubMed

Regulation

induced by DNA damage (LexA) PubMed

Regulatory mechanism

LexA: repression, PubMed, in lexA regulon

Biological materials

Mutant

MGNA-A926 (yerE::erm), available at the NBRP B. subtilis, Japan
BKE06600 (pcrB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTCATACGTTCCTCCAT,  downstream forward: _UP4_TAGTAAATTGTACGGATTTG
BKK06600 (pcrB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTCATACGTTCCTCCAT,  downstream forward: _UP4_TAGTAAATTGTACGGATTTG

References

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