pbuG

168

hypoxanthin/ guanine permease

Locus: BSU_06370

Molecular weight: 46.03 kDa

Isoelectric point: 8.81

Protein length: 440 aa

Gene length: 1323 bp

Function: hypoxanthine and guanine uptake

Product: hypoxanthin/ guanine permease

Essential: no

E.C.: null

Synonyms: pbuG, yebB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2252 (Galperin et al., 2021)

Gene

Coordinates: 694,662  695,984

The protein

Protein family

• xanthine/uracil permease family (according to UniProt)

Structure

• 8IRL (PDB) (from Arabidopsis thaliana, 32% identity) PubMed
• 2EEW (PDB) (the pbuG riboswitch, U47c mutant, bound to hypoxanthine)
• O34987 (AlphaFold)

Paralogous protein(s)

• PbuO

Localization

• membrane PubMed

Expression and Regulation

Operons

• Genes: pbuG

  Description: PubMed

  Regulation

  • repressed by hypoxanthine and guanine, PurR-independent, (G-box)PubMed

  Regulatory mechanism

  • PurR: RNA switch, (molecular inducer: PRPP) PubMed, in purR regulon
  • G-box: termination, (MgtE riboswitch) PubMed, in G-box

Biological materials

Mutant

• BKE06370 (pbuG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGCTATTTGACTCCCTTC,  downstream forward: _UP4_TAAGGAATGAAAAACCAGCT
• BKK06370 (pbuG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGCTATTTGACTCCCTTC,  downstream forward: _UP4_TAAGGAATGAAAAACCAGCT

References

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