bsuRC

168

component of the BsuM DNA restriction system

Locus: BSU_06110

Molecular weight: 54.17 kDa

Isoelectric point: 8.33

Protein length: 465 aa

Gene length: 1398 bp

Function: BsuM DNA restriction

Product: protection against foreign DNA

Essential: no

E.C.: null

Synonyms: ydjA

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 661,630 → 663,027

The protein

Catalyzed reaction/ biological activity

• Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates (according to UniProt)

Structure

• O34303 (AlphaFold)

Expression and Regulation

Operons

• Genes: bsuRA-bsuRB-bsuRC

  Description: PubMed

Biological materials

Mutant

• MGNA-C209 (ydjA::erm), available at the NBRP B. subtilis, Japan
• BKE06110 (ΔbsuRC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAACTTACTTGACTTATCCA,  downstream forward: _UP4_TAGTTCTAGAAGGTTAAAAG
• BKK06110 (ΔbsuRC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAACTTACTTGACTTATCCA,  downstream forward: _UP4_TAGTTCTAGAAGGTTAAAAG

References

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