thiL

168

thiamine-monophosphate kinase

Locus: BSU_05900

Molecular weight: 35.73 kDa

Isoelectric point: 5.04

Protein length: 325 aa

Gene length: 978 bp

Function: biosynthesis of thiamine

Product: thiamine-monophosphate kinase

Essential: no

E.C.: 2.7.4.16

Synonyms: thiL, ydiA, ydxA

Genomic Context

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List of homologs in different organisms, belongs to COG0611 (Galperin et al., 2021)

Gene

Coordinates: 640,662 → 641,639

The protein

Catalyzed reaction/ biological activity

• ATP + thiamine phosphate --> ADP + thiamine diphosphate (according to UniProt)

Protein family

• thiamine-monophosphate kinase family (single member, according to UniProt)

Structure

• 1VQV (PDB) (from Aquifex aeolicus, 33% similarity, 51% identity)
• O05514 (AlphaFold)

Expression and Regulation

Operons

• Genes: thiL-tsaE-tsaB-rimI-tsaD

  Description: PubMed

Biological materials

Mutant

• MGNA-C197 (ydiA::erm), available at the NBRP B. subtilis, Japan
• BKE05900 (ΔthiL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAACCAAAACCTCCTTT,  downstream forward: _UP4_TTTAAATAAGACGGGTGACT
• BKK05900 (ΔthiL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAACCAAAACCTCCTTT,  downstream forward: _UP4_TTTAAATAAGACGGGTGACT

References

Reviews

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Original Publications

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