gmuA

168

glucomannan-specific permease of the phosphotransferase system, EIIA of the PTS

Locus: BSU_05820

Molecular weight: 12.21 kDa

Isoelectric point: 5.63

Protein length: 110 aa

Gene length: 333 bp

Function: glucomannan uptake and phosphorylation

Product: glucomannan-specific lichenan-specific PTS, EIIA component

Essential: no

Synonyms: gmuA, ydhN

Genomic Context

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List of homologs in different organisms, belongs to COG1447 (Galperin et al., 2021)

Gene

Coordinates: 626,933  627,265

The protein

Protein family

• PTS permease, lactose family PubMed

Domains

• PTS EIIA domain type-3 (aa 9-107) (according to UniProt)

Structure

• 3K1S (PDB) (IIA(Cel) from B. anthracis, 42% identity)
• O05506 (AlphaFold)

Paralogous protein(s)

• LicA

Expression and Regulation

Operons

• Genes: gmuB-gmuA-gmuC-gmuD-gmuR-gmuE-gmuF-gmuG

  Description: PubMed

  Regulation

  • induced by cellobiose (GmuR) PubMed

  Regulatory mechanism

  • GmuR: repression, PubMed, in gmuR regulon
  • CcpA: repression, PubMed, in ccpA regulon
  • AbrB: activation, PubMed, in abrB regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-C189 (ydhN::erm), available at the NBRP B. subtilis, Japan
• BKE05820 (gmuA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATTGATTCACCATTAA,  downstream forward: _UP4_ATATAAAAAATCGGGGTGGA
• BKK05820 (gmuA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATTGATTCACCATTAA,  downstream forward: _UP4_ATATAAAAAATCGGGGTGGA

References

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