mhqO

168

hydroquinone-specific dioxygenase, confers resistence to methyl-hydroxyquinone

Locus: BSU_05490

Molecular weight: 34.90 kDa

Isoelectric point: 4.83

Protein length: 312 aa

Gene length: 939 bp

Function: resistence to methyl-hydroxyquinone

Product: hydroquinone-specific dioxygenase

Essential: no

Synonyms: mhqO, ydfO

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0346 (Galperin et al., 2021)

Gene

Coordinates: 597,114  598,052

The protein

Protein family

• extradiol ring-cleavage dioxygenase family (according to UniProt)

Domains

• 2 VOC domains (aa 7-131, aa 152-269) (according to UniProt)

Structure

• 3OAJ (PDB)
• P96693 (AlphaFold)

Paralogous protein(s)

• MhqA, MhqE

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: mhqO-mhqP

  Description: PubMed

  Regulation

  • induced by catechol and 2-methylhydroquinone (MhqR) PubMed

  Regulatory mechanism

  • MhqR: repression, PubMed, in mhqR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigB: sigma factor, PubMed, in sigB regulon

• Genes: mhqN-mhqO-mhqP

  Description: PubMed

  Regulation

  • induced by catechol and 2-methylhydroquinone (MhqR) PubMed

  Regulatory mechanism

  • MhqR: repression, PubMed, in mhqR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-C154 (ydfO::erm), available at the NBRP B. subtilis, Japan
• BKE05490 (mhqO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTAGCCATTTTTATCCGCT,  downstream forward: _UP4_TGATTTGACACATAAAAAAT
• BKK05490 (mhqO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTAGCCATTTTTATCCGCT,  downstream forward: _UP4_TGATTTGACACATAAAAAAT

References

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