ydeF

168

similar to transcriptional regulator (MocR/ GabR family)

Locus: BSU_05180

Molecular weight: 51.64 kDa

Isoelectric point: 6.09

Protein length: 465 aa

Gene length: 1398 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ydeF

Genomic Context

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List of homologs in different organisms, belongs to COG1167 (Galperin et al., 2021)

Gene

Coordinates: 564,704 → 566,101

The protein

Protein family

• MocR/ GabR family PubMed
• class-I pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Domains

• N-terminal DNA-binding helix-turn-helix motif; C-terminal domain is homologous to PLP-binding large domain of aminotransferases.
• HTH gntR-type domain (aa 22-90) (according to UniProt)

Cofactors

• PLP

Structure

• 4MGR (PDB) (GabR, 23% identity) PubMed
• P96663 (AlphaFold)

Biological materials

Mutant

• MGNA-C082 (ydeF::erm), available at the NBRP B. subtilis, Japan
• BKE05180 (ΔydeF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATACGCATCCTTTTTC,  downstream forward: _UP4_TAATATTGTTTGCCATACAA
• BKK05180 (ΔydeF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATACGCATCCTTTTTC,  downstream forward: _UP4_TAATATTGTTTGCCATACAA

References

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