yddN

168

similar to alkanal monooxygenase

Locus: BSU_05040

Molecular weight: 37.88 kDa

Isoelectric point: 5.19

Protein length: 339 aa

Gene length: 1020 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yddN

Genomic Context

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List of homologs in different organisms, belongs to COG2141 (Galperin et al., 2021)

Gene

Coordinates: 550,240  551,259

The protein

Cofactors

• FMN or FAD PubMed

Structure

• 4US5 (PDB) (from Streptomyces bottropensis, 30% identity)
• P96651 (AlphaFold)

Paralogous protein(s)

• YvbT
• YceB, (33,6%)

Expression and Regulation

Operons

• Genes: yddN

  Description: PubMed

Biological materials

Mutant

• MGNA-C124 (yddN::erm), available at the NBRP B. subtilis, Japan
• BKE05040 (yddN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATTTCACCTCTAAAAT,  downstream forward: _UP4_TAGAAATCGAGTCAGTTGTA
• BKK05040 (yddN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATTTCACCTCTAAAAT,  downstream forward: _UP4_TAGAAATCGAGTCAGTTGTA