xis

168

excisionase

Locus: BSU_04830

Molecular weight: 7.17 kDa

Isoelectric point: 9.64

Protein length: 64 aa

Gene length: 195 bp

Function: excision of the conjugative transposon ICEBs1 from the trnS-leu2 locus

Product: excisionase

Essential: no

E.C.: null

Synonyms: xis, ydzC, sacV

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 531,787 → 531,981

The protein

Catalyzed reaction/ biological activity

• excision of conjugative transposon PubMed

Structure

• O31490 (AlphaFold)

Additional information

• Was reported to be involved in the regulation of the levansucrase gene (''sacB'')

Expression and Regulation

Operons

• Genes: xis-ydzL-devI-ydcP-ydcQ-nicK-S172-ramS-ramT-ramA-conB-conC-conD-conE-yddF-conG-cwlT-yddI-yddJ

  Description: PubMed

  Regulatory mechanism

  • ImmR: repression, PubMed, in immR regulon

Biological materials

Mutant

• BKE04830 (Δxis::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAATATCACCTCCTCGTT,  downstream forward: _UP4_AAAGGCCCAAAATCTCAAAC
• BKK04830 (Δxis::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAATATCACCTCCTCGTT,  downstream forward: _UP4_AAAGGCCCAAAATCTCAAAC

References

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