ssdC

168

MucB/RseB-like protein, affects the assembly of the spore cortex and spore shape

Locus: BSU_04630

Molecular weight: 42.03 kDa

Isoelectric point: 9.6

Protein length: 338 aa

Gene length: 1017 bp

Function: assembly of the spore cortex

Product: spore shape determinant C

Essential: no

Synonyms: ydcC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2834 (Galperin et al., 2021)

Gene

Coordinates: 516,241 → 517,257

Phenotypes of a mutant

• defect in sporulation PubMed
• some production of small spores, reduced SigG activity PubMed
• forespores are rounder than those of the wild type PubMed
• produces spores with an abnormal-looking cortex, abolishing cortex synthesis in the ssdC mutant largely supresses the spore shape defect PubMed

The protein

Domains

• N-terminal transmembrane segment and a large extracellular C-terminal-domain PubMed

Structure

• P96619 (AlphaFold)

Modification

• phosphorylated on Thr-197 and Thr-201 PubMed

Localization

• cell membrane PubMed
• forms a discontinuous, dynamic ring-like structure in the peripheral membrane of the mother cell, near the mother cell proximal pole of the forespore PubMed
• localization depends on SpoVM, SpoIVA, SpoVID, and SafA PubMed

Expression and Regulation

Operons

• Genes: ssdC

  Description: PubMed

  Regulation

  • expressed in the mother cell (SigE) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-C122 (ydcC::erm), available at the NBRP B. subtilis, Japan
• BKE04630 (ΔssdC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACCCCTTTTTCTCAATTG,  downstream forward: _UP4_TAACCGCCAAAGGCCAAACA
• BKK04630 (ΔssdC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTATGATAACCTCCTTT,  downstream forward: _UP4_TAGTCTGCATATTAGGGAAA

References

Loading