dctR

168

two-component response regulator, regulation of the dctS-dctR-dctP operon

Locus: BSU_04460

Molecular weight: 25.39 kDa

Isoelectric point: 8.6

Protein length: 226 aa

Gene length: 681 bp

Function: regulation of the dctS-dctR-dctP operon

Product: two-component response regulator

Essential: no

Synonyms: dctR, ydbG

Genomic Context

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Categories containing this gene/protein

Metabolism, Carbon metabolism, Utilization of specific carbon sources, Utilization of organic acids

Information processing, Regulation of gene expression, Transcription factors and their control, Two-component system response regulators

Groups of genes, Phosphoproteins, Phosphorylation on an Asp residue

List of homologs in different organisms, belongs to COG4565 (Galperin et al., 2021)

Gene

Coordinates: 499,365  500,045

The protein

Domains

Response regulatory domain (aa 7-123) (according to UniProt)

Structure

4HYE (PDB) (Spr1814 from Streptococcus pneumoniae, 30% identity) PubMed
P96602 (AlphaFold)

Modification

phosphorylated by DctS on an Asp residue

Effectors of protein activity

phosphorylation likely affects DNA-binding activity

Localization

cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

Genes: dctS-dctR-dctP

Description: PubMed

Regulation

part of the iron sparing response, strong down-regulation in a'' fur'' mutant (Fur, FsrA) PubMed

Biological materials

Mutant

BKE04460 (dctR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCAATGAGCAGAACCTTCC,  downstream forward: _UP4_TAAACCTGAGACCAAAAAGA
BKK04460 (dctR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCAATGAGCAGAACCTTCC,  downstream forward: _UP4_TAAACCTGAGACCAAAAAGA

References

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