mutT

168

antimutator protein, has RNA pyrophosphohydrolase activity

Locus: BSU_04330

Molecular weight: 16.26 kDa

Isoelectric point: 8.59

Protein length: 149 aa

Gene length: 450 bp

Function: DNA repair, protection against oxidative stress

Product: antimutator protein

Essential: no

Synonyms: mutT

Genomic Context

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List of homologs in different organisms, belongs to COG1051 (Galperin et al., 2021)

Gene

Coordinates: 488,314  488,763

The protein

Catalyzed reaction/ biological activity

• 8-oxo-dGTP + H₂O --> 8-oxo-dGMP + diphosphate + H⁺ (according to UniProt)

Protein family

• Nudix hydrolase (according to UniProt)

Domains

• Nudix hydrolase domain (aa 1-123) (according to UniProt)

Cofactors

• Mn²⁺ PubMed

Structure

• 1VC8 (PDB) (N-terminal 100 amino acids of Ap6A hydrolase from Thermus thermophilus, 40% identity)
• P96590 (AlphaFold)

Biological materials

Mutant

• MGNA-C093 (mutT::erm), available at the NBRP B. subtilis, Japan
• JH642 and its derivatives: natural deletion of the 18 kb ydzA-mntH region encompassing the genes ydzA-topB-ydaJ-ydaK-ydaL-ydaM-ydaN-kimA-mutT-ydaP-ydzK-mntH PubMed
• BKE04330 (mutT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCCCCATACCTCCTTTTC,  downstream forward: _UP4_TGATTCAGGTCTCCGGGTTT
• BKK04330 (mutT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCCCCATACCTCCTTTTC,  downstream forward: _UP4_TGATTCAGGTCTCCGGGTTT

References

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