ydaM

168

general stress protein, synthesis of extracellular polysaccharide

Locus: BSU_04300

Molecular weight: 49.61 kDa

Isoelectric point: 9.73

Protein length: 420 aa

Gene length: 1263 bp

Function: synthesis of extracellular polysaccharide

Product: unknown

Essential: no

Synonyms: ydaM

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1215 (Galperin et al., 2021)

Gene

Coordinates: 482,577  483,839

The protein

Protein family

• glycosyltransferase 2 family PubMed

Structure

• P96587 (AlphaFold)

Localization

• cell membrane, forms clusters at the cell poles and septa (with YdaK and YdaN) PubMed

Expression and Regulation

Operons

• Genes: ydaJ-ydaK-ydaL-ydaM-ydaN

  Description: PubMed

  Regulation

  • expressed during stress (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• MGNA-C076 (ydaM::erm), available at the NBRP B. subtilis, Japan
• JH642 and its derivatives: natural deletion of the 18 kb ydzA-mntH region encompassing the genes ydzA-topB-ydaJ-ydaK-ydaL-ydaM-ydaN-kimA-mutT-ydaP-ydzK-mntH PubMed
• BKE04300 (ydaM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ACTTAGCGAGATAAAGAACA,  downstream forward: _UP4_CAACATAAAAGCGGGTGACC
• BKK04300 (ydaM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ACTTAGCGAGATAAAGAACA,  downstream forward: _UP4_CAACATAAAAGCGGGTGACC

References

Reviews

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Original Publications

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