ydaL

168

general stress protein, synthesis of extracellular polysaccharide

Locus: BSU_04290

Molecular weight: 63.68 kDa

Isoelectric point: 6.68

Protein length: 569 aa

Gene length: 1710 bp

Function: synthesis of extracellular polysaccharide

Product: unknown

Essential: no

Synonyms: ydaL

Genomic Context

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List of homologs in different organisms, belongs to COG5298 (Galperin et al., 2021)

Gene

Coordinates: 480,875  482,584

The protein

Protein family

• carbohydrate esterase 4 superfamily PubMed

Structure

• O31487 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: ydaJ-ydaK-ydaL-ydaM-ydaN

  Description: PubMed

  Regulation

  • expressed during stress (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• MGNA-C075 (ydaL::erm), available at the NBRP B. subtilis, Japan
• JH642 and its derivatives: natural deletion of the 18 kb ydzA-mntH region encompassing the genes ydzA-topB-ydaJ-ydaK-ydaL-ydaM-ydaN-kimA-mutT-ydaP-ydzK-mntH PubMed
• BKE04290 (ydaL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACGCATAGCAATGTGA,  downstream forward: _UP4_CGAATTTTTAAGGAGAGAAG
• BKK04290 (ydaL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACGCATAGCAATGTGA,  downstream forward: _UP4_CGAATTTTTAAGGAGAGAAG

References

Reviews

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Original Publications

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