ycsD

168

ß-hydroxyacyl-(acyl carrier protein) dehydratase

Locus: BSU_04030

Molecular weight: 14.11 kDa

Isoelectric point: 4.69

Protein length: 130 aa

Gene length: 393 bp

Function: fatty acid biosynthesis

Product: ß-hydroxyacyl-ACP dehydratase

Essential: no

Synonyms: ycsD

Genomic Context

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Categories containing this gene/protein

Metabolism, Lipid metabolism, Biosynthesis of lipids, Biosynthesis of fatty acids

List of homologs in different organisms, belongs to COG0764 (Galperin et al., 2021)

Gene

Coordinates: 455,346  455,738

The protein

Protein family

thioester dehydratase family (with YwpB, according to UniProt)

Structure

4I83 (PDB)((3r)-hydroxymyristoyl-ACP dehydratase from Neisseria meningitidis, 34% identity)
P42961 (AlphaFold)

Paralogous protein(s)

YwpB

Expression and Regulation

Operons

Genes: ycsD

Description: PubMed

Biological materials

Mutant

MGNA-C021 (ycsD::erm), available at the NBRP B. subtilis, Japan
BKE04030 (ycsD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTATATCTCCTTTTTT,  downstream forward: _UP4_TGAAAAAAACCCTTCACAAC
BKK04030 (ycsD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTATATCTCCTTTTTT,  downstream forward: _UP4_TGAAAAAAACCCTTCACAAC

References

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