phrC

168

response regulator aspartate phosphatase (RapC) regulator / competence and sporulation stimulating factor (CSF)

locus: BSU_03780

Molecular weight: 4.06 kDa

pI: 8.16

Protein length: 40 aa

Gene length: 123 bp

function: control of ComA activity

product: phosphatase (RapC) regulator / competence and sporulation stimulating factor (CSF)

essential: no

ec: null

synonyms: phrC

List of homologs in different organisms

Gene

Coordinates: 429,963 → 430,085

The protein

Catalyzed reaction/ biological activity

• binds to [protein|242632B27E0E3AB15777E9FBA3FC746D00CE97F9|rapC], this results in the inability of [protein|242632B27E0E3AB15777E9FBA3FC746D00CE97F9|rapC] to interact with [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA] [Pubmed|12950917]

Protein family

• [wiki|phr family] (according to UniProt)

Structure

• [PDB|4GYO] (complex [protein|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|rapJ]-[protein|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|phrC]) [Pubmed|23526881]
• [AF|P94416]

Expression and Regulation

Operons

• genes: [gene|242632B27E0E3AB15777E9FBA3FC746D00CE97F9|rapC]-[gene|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|phrC]

  description: [Pubmed|10464187]

  regulation

  • expressed at high cell density ([protein|search|ComA]) [Pubmed|16091051]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|10464187], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
  • [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA]: activation, ([protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA]) [Pubmed|16091051], in [regulon|protein:7832489F11F606BCF637EC23BAAB41AD10237EBB|comA regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10464187], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|10464187], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]

• genes: [gene|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|phrC]

  description: [Pubmed|10464187]

  regulation

  • expressed at high cell density ([protein|search|ComA]) [Pubmed|16091051]

Biological materials

Mutant

• BKE03780 (Δ[gene|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|phrC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03780 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ACTTAGATTTCAATTTCATA,  downstream forward: _UP4_TAAGAACAAGCCCCTTCTCA
• BKK03780 (Δ[gene|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|phrC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03780 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ACTTAGATTTCAATTTCATA,  downstream forward: _UP4_TAAGAACAAGCCCCTTCTCA

References

Reviews

• 22024380,37042030

Original Publications

• 12950917,16816200,19202088,18375560,11267663,10464187,12850135,16091051,23526881,22511326