ycxD

168

similar to transcriptional regulator (MocR/ GabR family)

Locus: BSU_03560

Molecular weight: 50.59 kDa

Isoelectric point: 8.57

Protein length: 444 aa

Gene length: 1335 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ycxD

Genomic Context

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List of homologs in different organisms, belongs to COG1167 (Galperin et al., 2021)

Gene

Coordinates: 406,131  407,465

The protein

Protein family

• MocR/ GabR family PubMed
• class-I pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Domains

• N-terminal DNA-binding helix-turn-helix motif; C-terminal domain is homologous to PLP-binding large domain of aminotransferases.
• HTH gntR-type domain (aa 1-69) (according to UniProt)

Cofactors

• PLP

Structure

• 4MGR (PDB) (GabR, 27% identity) PubMed
• Q08792 (AlphaFold)

Biological materials

Mutant

• BKE03560 (ycxD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCCTTCTCCCCCTGTTT,  downstream forward: _UP4_GGTGTCAAGCTGTTGATGAG
• BKK03560 (ycxD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCCTTCTCCCCCTGTTT,  downstream forward: _UP4_GGTGTCAAGCTGTTGATGAG

Expression vectors

• pGP3930 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab

References

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