hxlB

168

6-phospho-3-hexuloisomerase

Locus: BSU_03450

Molecular weight: 19.82 kDa

Isoelectric point: 5.79

Protein length: 185 aa

Gene length: 558 bp

Function: ribulose monophosphate pathway for formaldehyde fixation

Product: 6-phospho-3-hexuloisomerase

Essential: no

E.C.: 5.3.1.27

Synonyms: hxlB, yckF

Genomic Context

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List of homologs in different organisms, belongs to COG0794 (Galperin et al., 2021)

Gene

Coordinates: 374,603 → 375,160

The protein

Catalyzed reaction/ biological activity

• D-arabino-hex-3-ulose 6-phosphate --> β-D-fructose 6-phosphate (according to UniProt)

Protein family

• SIS family (single member, according to UniProt)

Domains

• SIS domain (aa 29-172) (according to UniProt)

Structure

• 1M3S (PDB) PubMed
• P42404 (AlphaFold)

Expression and Regulation

Operons

• Genes: hxlA-hxlB

  Description: PubMed

  Regulation

  • induction by formaldehyde (HxlR) PubMed

  Regulatory mechanism

  • HxlR: activation, PubMed, in hxlR regulon

Biological materials

Mutant

• MGNA-C056 (yckF::erm), available at the NBRP B. subtilis, Japan
• BKE03450 (ΔhxlB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTATTCAGTCGTTTTCATCG,  downstream forward: _UP4_TAGCATCACACAACCGGCCT
• BKK03450 (ΔhxlB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTATTCAGTCGTTTTCATCG,  downstream forward: _UP4_TAGCATCACACAACCGGCCT

References

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