putR

168

transcriptional activator of the proline utilization operon putB-putC-putP

Locus: BSU_03230

Molecular weight: 47.67 kDa

Isoelectric point: 5.37

Protein length: 411 aa

Gene length: 1236 bp

Function: regulation of proline utilization

Product: transcriptional activator (PucR family)

Essential: no

Synonyms: putR, ycgP, prcR

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2508 (Galperin et al., 2021)

Gene

Coordinates: 348,724 → 349,959

Phenotypes of a mutant

• no growth with proline as single source of carbon or nitrogen PubMed

The protein

Catalyzed reaction/ biological activity

• transcription activation of the proline utilization operon ''putB-putC-putP'' in the presence of proline  PubMed

Protein family

• PucR family
• CdaR family (according to UniProt)

Cofactors

• proline acts as co-activator  PubMed

Structure

• P94393 (AlphaFold)

Effectors of protein activity

• proline activates PutR  PubMed

Expression and Regulation

Operons

• Genes: putR

  Description: PubMed

  Regulation

  • constitutively expressed PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE03230 (ΔputR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACCTTTTTCTCCTATAC,  downstream forward: _UP4_TAAAAAAGAAACCAGTACTT
• BKK03230 (ΔputR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACCTTTTTCTCCTATAC,  downstream forward: _UP4_TAAAAAAGAAACCAGTACTT

References

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