PutP

• Description: high affinity proline permease
  
  

Gene name	putP
Synonyms	ycgO
Essential	no
Product	high affinity proline permease
Function	proline uptake
Gene expression levels in SubtiExpress: putP
MW, pI	48 kDa, 8.619
Gene length, protein length	1347 bp, 449 aa
Immediate neighbours	putC, putR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids, utilization of amino acids membrane proteins

This gene is a member of the following regulons

PutR regulon

The gene

Basic information

• Locus tag: BSU03220

Phenotypes of a mutant

• no growth with proline as single source of carbon or nitrogen PubMed

Database entries

• BsubCyc: BSU03220

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • uptake of proline coupled to the uptake of sodium ions

• Protein family: sodium solute symporter family (SSS; TC2A.21)

• Paralogous protein(s): OpuE

Extended information on the protein

• Kinetic information:
  • K(M) for proline: 8 +/- 2 µM PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU03220

• Structure:

• UniProt: P94392

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
  • putB-putC-putP PubMed
  • putP (internal promoter) PubMed

• Expression browser: putP PubMed

• Sigma factor:
  • putB: SigA PubMed

• Regulation:
  • induced in the presence of proline (PutR) PubMed

• Regulatory mechanism:
  • PutR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • available in Erhard Bremer's lab PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: