ycdG

168

general stress protein, similar to oligo-1,6-glucosidase

Locus: BSU_02840

Molecular weight: 65.64 kDa

Isoelectric point: 5.21

Protein length: 561 aa

Gene length: 1686 bp

Function: unknown

Product: unknown

Essential: no

E.C.: 3.2.1.10

Synonyms: ycdG

Genomic Context

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List of homologs in different organisms, belongs to COG0366 (Galperin et al., 2021)

Gene

Coordinates: 306,459  308,144

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of (16)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose (according to UniProt)

Protein family

• glycosyl hydrolase 13 family (according to UniProt)

Structure

• 1UOK (PDB) (from Bacillus Cereus, 54% identity)
• O34364 (AlphaFold)

Paralogous protein(s)

• YugT, TreA, MalL

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ycdF-ycdG

  Description: PubMed

  Regulation

  • induced by stress (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• BKE02840 (ycdG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCCGCAATCCCCCTGTT,  downstream forward: _UP4_TAATGATTGAAGTAGCCCGG
• BKK02840 (ycdG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCCGCAATCCCCCTGTT,  downstream forward: _UP4_TAATGATTGAAGTAGCCCGG

References

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