ycdF

168

general stress protein, similar to glucose 1-dehydrogenase, survival of ethanol stress and low temperatures

Locus: BSU_02830

Molecular weight: 27.62 kDa

Isoelectric point: 5.74

Protein length: 258 aa

Gene length: 777 bp

Function: survival of ethanol stress and low temperatures

Product: unknown

Essential: no

E.C.: 1.1.1.47

Synonyms: ycdF

Genomic Context

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List of homologs in different organisms, belongs to COG1028 (Galperin et al., 2021)

Gene

Coordinates: 305,658 → 306,434

The protein

Catalyzed reaction/ biological activity

• Beta-D-glucose + NAD(P)⁺ --> D-glucono-1,5-lactone + NAD(P)H (according to UniProt)
• Beta-D-glucose + NAD⁺ --> D-glucono-1,5-lactone + NADH (according to UniProt)

Protein family

• Short-chain dehydrogenases/reductases (SDR) family (according to UniProt)

Structure

• 1GEE (PDB) (from Bacillus megaterium, 56% identity)
• P80869 (AlphaFold)

Paralogous protein(s)

• YxbG, BacC, YmfI, FabG, YoxD
• Gdh, (53%)
• FadH, (31.9%)
• YhdF, (34.3%)
• KduD, (34.3%)
• YdaD, (69%)
• YkvO, (31,9%)
• YhxC, (32,4%),
• YhxD, (31,1%)
• FabL, (31.6%)

Expression and Regulation

Operons

• Genes: ycdF-ycdG

  Description: PubMed

  Regulation

  • induced by stress (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• MGNA-C046 (ycdF::erm), available at the NBRP B. subtilis, Japan
• BKE02830 (ΔycdF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACTTGATATCCTCCTCT,  downstream forward: _UP4_TAGAAGACAAAACAGGGGGA
• BKK02830 (ΔycdF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACTTGATATCCTCCTCT,  downstream forward: _UP4_TAGAAGACAAAACAGGGGGA

References

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