pcp

168

pyrrolidone-carboxylate peptidase

Locus: BSU_02650

Molecular weight: 23.63 kDa

Isoelectric point: 6.42

Protein length: 215 aa

Gene length: 648 bp

Function: removal of the N-terminal pyroglutamyl group from peptides

Product: pyrrolidone-carboxylate peptidase

Essential: no

E.C.: 3.4.11.8

Synonyms: pcp

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2039 (Galperin et al., 2021)

Gene

Coordinates: 286,773  287,420

The protein

Catalyzed reaction/ biological activity

• Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro (according to UniProt)

Protein family

• peptidase C15 family (single member, according to UniProt)

Structure

• 1AUG (PDB) (from B. amyloliquefaciens, 72% identity) PubMed
• P28618 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: pcp

  Description: PubMed

Biological materials

Mutant

• BKE02650 (pcp::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTCCCATCTCCTTTTT,  downstream forward: _UP4_CACTAAGCGGCCGCCGCTGT
• BKK02650 (pcp::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTCCCATCTCCTTTTT,  downstream forward: _UP4_CACTAAGCGGCCGCCGCTGT

References

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