ycbK

168

similar to efflux system

Locus: BSU_02540

Molecular weight: 33.81 kDa

Isoelectric point: 9.57

Protein length: 312 aa

Gene length: 939 bp

Function: unknown

Product: unknown

Essential: no

E.C.: null

Synonyms: ycbK

Genomic Context

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Categories containing this gene/protein

Cellular processes, Transporters, Transporters/ other, Other transporters

Groups of genes, Membrane proteins

List of homologs in different organisms, belongs to COG0697 (Galperin et al., 2021)

Gene

Coordinates: 277,342 → 278,280

The protein

Protein family

EamA transporter family (according to UniProt)

Domains

EamA domain (aa 173-303) (according to UniProt)

Structure

P42243 (AlphaFold)

Localization

cell membrane (according to UniProt)

Expression and Regulation

Operons

Genes: rtpA-ycbK

Description: PubMed

Regulation

''rtpA'': induced by tryptophan limitation (T-box in the mRNA leader) PubMed

Regulatory mechanism

T-box: RNA switch, MtrB PubMed, in T-box

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Other regulations

MtrB: translation inhibition

Biological materials

Mutant

MGNA-C033 (ycbK::erm), available at the NBRP B. subtilis, Japan
BKE02540 (ΔycbK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCAGCTCAGCTTTTCT,  downstream forward: _UP4_TAATTTTTATAAAACACACA
BKK02540 (ΔycbK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCAGCTCAGCTTTTCT,  downstream forward: _UP4_TAATTTTTATAAAACACACA

References

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