ycbK

168

similar to efflux system

Locus: BSU_02540

Molecular weight: 33.81 kDa

Isoelectric point: 9.57

Protein length: 312 aa

Gene length: 939 bp

Function: unknown

Product: unknown

Essential: no

E.C.: null

Synonyms: ycbK

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0697 (Galperin et al., 2021)

Gene

Coordinates: 277,342 → 278,280

The protein

Protein family

• EamA transporter family (according to UniProt)

Domains

• EamA domain (aa 173-303) (according to UniProt)

Structure

• P42243 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: rtpA-ycbK

  Description: PubMed

  Regulation

  • ''rtpA'': induced by tryptophan limitation (T-box in the mRNA leader) PubMed

  Regulatory mechanism

  • T-box: RNA switch, MtrB PubMed, in T-box

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Other regulations

• MtrB: translation inhibition

Biological materials

Mutant

• MGNA-C033 (ycbK::erm), available at the NBRP B. subtilis, Japan
• BKE02540 (ΔycbK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCAGCTCAGCTTTTCT,  downstream forward: _UP4_TAATTTTTATAAAACACACA
• BKK02540 (ΔycbK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCAGCTCAGCTTTTCT,  downstream forward: _UP4_TAATTTTTATAAAACACACA

References

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