ybfF

168

unknown

Locus: BSU_02190

Molecular weight: 35.16 kDa

Isoelectric point: 9.67

Protein length: 303 aa

Gene length: 912 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ybfF

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 238,644 → 239,555

The protein

Structure

• O31446 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: ybfG-ybfF-ybfE

  Description: PubMed

  Regulation

  • the mRNA is processed between ybfG and ybfF by RNase Y, this requires the RicA-RicF-RicT complex PubMed

Biological materials

Mutant

• MGNA-B967 (ybfF::erm), available at the NBRP B. subtilis, Japan
• BKE02190 (ΔybfF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTGATAAACCTCCAA,  downstream forward: _UP4_TAATTTTGTTTCATCCTTAC
• BKK02190 (ΔybfF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTGATAAACCTCCAA,  downstream forward: _UP4_TAATTTTGTTTCATCCTTAC

References

Research papers

DeLoughery A, Lalanne JB, Losick R, Li GW Maturation of polycistronic mRNAs by the endoribonuclease RNase Y and its associated Y-complex in . Proceedings of the National Academy of Sciences of the United States of America. 2018 May 24; . pii:201803283. doi:10.1073/pnas.1803283115. PMID:29794222