GlpQ

• Description: glycerolphosphate diester phosphodiesterase
  
  

Gene name	glpQ
Synonyms	ybeD
Essential	no
Product	glycerolphosphate diester phosphodiesterase
Function	glycerol-3-phosphate utilization
Gene expression levels in SubtiExpress: glpQ
MW, pI	32 kDa, 9.263
Gene length, protein length	879 bp, 293 aa
Immediate neighbours	ybeC, glpT
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, utilization of lipids

This gene is a member of the following regulons

CcpA regulon, GlpP regulon, PhoP regulon

The gene

Basic information

• Locus tag: BSU02130

Database entries

• BsubCyc: BSU02130

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: A glycerophosphodiester + H₂O = an alcohol + sn-glycerol 3-phosphate (according to Swiss-Prot)

• Protein family: glycerophosphoryl diester phosphodiesterase family (according to Swiss-Prot)

• Paralogous protein(s): YhdW, YqiK

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide) PubMed

Database entries

• BsubCyc: BSU02130

• Structure: 2PZ0 (the enzyme from Thermoanaerobacter tengcongensis) PubMed

• UniProt: P37965

• KEGG entry: [3]

• E.C. number: 3.1.4.46

Expression and regulation

• Operon:
  • glpT-glpQ PubMed
  • glpQ

• Expression browser: glpQ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (6.2-fold) (CcpA) PubMed
  • induced by glycerol (GlpP) PubMed
  • glpQ: induced by phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression
  • GlpP: transcriptional antitermination via a protein-dependent RNA switch
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Daniela Corda, Maria G Mosca, Noriyasu Ohshima, Laura Grauso, Noriyuki Yanaka, Stefania Mariggiò
The emerging physiological roles of the glycerophosphodiesterase family.
FEBS J: 2014, 281(4);998-1016
[PubMed:24373430] [WorldCat.org] [DOI] (I p)

Original publications

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Liang Shi, Jun-Feng Liu, Xiao-Min An, Dong-Cai Liang
Crystal structure of glycerophosphodiester phosphodiesterase (GDPD) from Thermoanaerobacter tengcongensis, a metal ion-dependent enzyme: insight into the catalytic mechanism.
Proteins: 2008, 72(1);280-8
[PubMed:18214974] [WorldCat.org] [DOI] (I p)

H Antelmann, C Scharf, M Hecker
Phosphate starvation-inducible proteins of Bacillus subtilis: proteomics and transcriptional analysis.
J Bacteriol: 2000, 182(16);4478-90
[PubMed:10913081] [WorldCat.org] [DOI] (P p)

R P Nilsson, L Beijer, B Rutberg
The glpT and glpQ genes of the glycerol regulon in Bacillus subtilis.
Microbiology (Reading): 1994, 140 ( Pt 4);723-30
[PubMed:8012593] [WorldCat.org] [DOI] (P p)