rsiW

168

anti-SigW

Locus: BSU_01740

Molecular weight: 23.19 kDa

Isoelectric point: 6.65

Protein length: 208 aa

Gene length: 627 bp

Function: control of SigW activity

Product: anti-SigW

Essential: no

Synonyms: rsiW, ybbM

Genomic Context

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List of homologs in different organisms, belongs to COG5662 (Galperin et al., 2021)

Gene

Coordinates: 195,426  196,052

The protein

Protein family

• zinc-associated anti-sigma factor (ZAS) superfamily (with YlaD, according to UniProt)
• anti-sigma-W factor family (single member, according to UniProt)

Domains

• The cytoplasmic domain is able to inactivate SigW and the extracellular and transmembrane domains are crucial for sensing and transducing the signal that triggers SigW activation (PubMed:15130127). The N-terminus binds the zinc ion and is followed by a long helix (about residues 40-80) that fits into a hydrophobic surface groove on SigW, probably blocking its ability to interact with the -10 and -35 promoter elements (PubMed:28319136).

Structure

• 5WUQ (PDB) (the cytoplasmic domain in complex with SigW) PubMed
• Q45588 (AlphaFold)

Effectors of protein activity

• degraded by RasP and PrsW under conditions of alkali shock or in the presence of antimicrobial peptides, respectively. This results in the release of SigW

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: sigW-rsiW

  Description: PubMed

  Regulation

  • induced under conditions of alkali shock or in the presence of the toxin SdpC (SigW) PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon

  Sigma factors

  • SigW: sigma factor, PubMed, in sigW regulon

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• MGNA-B953 (ybbM::erm), available at the NBRP B. subtilis, Japan
• BKE01740 (rsiW::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGGACAGCTCATTTCATCAC,  downstream forward: _UP4_TAAAGCGCGTTAGCCGCTTT
• BKK01740 (rsiW::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGGACAGCTCATTTCATCAC,  downstream forward: _UP4_TAAAGCGCGTTAGCCGCTTT

Labs working on this gene/protein

• Thomas Wiegert, University of Bayreuth, Germany Homepage

References

Reviews

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Original Publications

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