cwlD

168

N-acetylmuramoyl-L-alanine amidase, spore cortex peptidoglycan synthesis

locus: BSU_01530

Molecular weight: 26.86 kDa

pI: 9.79

Protein length: 237 aa

Gene length: 714 bp

function: spore cortex peptidoglycan synthesis

product: N-acetylmuramoyl-L-alanine amidase

essential: no

ec: 3.5.1.28

synonyms: cwlD

List of homologs in different organisms, belongs to COG0860 (Galperin et al., 2021)

Gene

Coordinates: 156,612 → 157,325

The protein

Catalyzed reaction/ biological activity

• formation of muramic acid delta-lacton in spore cortex peptidoglycan [Pubmed|14679227]
• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

• [wiki|N-acetylmuramoyl-L-alanine amidase 3 family] (according to UniProt)

[wiki|Domains]

• contains an amidase_3 domain (like [protein|5CDF32B0E78F01C696364555E3F0BC8DF2AA2089|cwlC], [protein|6A21293823151C6980BF52B31A4B249A8440F2E1|lytC], [protein|0AD75864794E597AA9160969ADE6FE0C7ED07B9F|yqiI], [protein|BE34A9CE3AF99E8880FC24E34E5D79A1627260AE|yrvJ])
• [wiki|MurNAc-LAA domain] (aa 43-226) (according to UniProt)

Structure

• [PDB|7RAG] (from Clostridium difficile, complex with [protein|search|GerS], 40.4% identity) [pubmed|34570752]
• [AF|P50864]

[wiki|Localization]

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|64786CC240BC208571AA3B3D28B5167FEF8CD428|ybaK]-[gene|B09FB626274B81F00A4FB42D188E089095343182|cwlD]

  description: [Pubmed|7559346]

  regulation

  • expressed during sporulation ([protein|search|SigG]) [Pubmed|7559346]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|15699190,7559346], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]

• genes: [gene|B09FB626274B81F00A4FB42D188E089095343182|cwlD]

  description: [Pubmed|7559346]

  regulation

  • expressed during sporulation ([protein|search|SigG], [protein|search|SigE]) [Pubmed|7559346]

Biological materials

Mutant

• BKE01530 (Δ[gene|B09FB626274B81F00A4FB42D188E089095343182|cwlD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01530 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCCTTCCCCTCCCGCTT,  downstream forward: _UP4_TAATGGAGGGTTTTCTTGTG
• BKK01530 (Δ[gene|B09FB626274B81F00A4FB42D188E089095343182|cwlD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01530 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCCTTCCCCTCCCGCTT,  downstream forward: _UP4_TAATGGAGGGTTTTCTTGTG

References

• 12646705,10498740,7559346,14679227,16267290,22123250,24405365,35638784,34570752,37021938