Difference between revisions of "YabT"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00660&redirect=T BSU00660]
  
 
* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00660&redirect=T BSU00660]
  
 
* '''Structure:'''
 
* '''Structure:'''

Revision as of 12:47, 2 April 2014

  • Description: Ser/Thr kinase, controls DNA integrity during spore development

Gene name yabT
Synonyms
Essential no
Product Ser/Thr kinase
Function control of DNA integrity during spore development
Gene expression levels in SubtiExpress: yabT
Interactions involving this protein in SubtInteract: YabT
MW, pI 37 kDa, 9.943
Gene length, protein length 1014 bp, 338 aa
Immediate neighbours yabS, tilS
Sequences Protein DNA DNA_with_flanks
Genetic context
YabT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YabT expression.png















Categories containing this gene/protein

protein modification, sporulation proteins

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

  • Locus tag: BSU00660

Phenotypes of a mutant

  • increased sensitivity to DNA damage during spore development PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
    • phosphorylation of RecA on Ser-2 PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • autophosphorylation is stimulated by non-specific binding to DNA PubMed
  • Localization:
    • colocalizes strongly with the septal membrane separating the mother cells from the forespore PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation: expressed early during sporulation in the forespore (SigF) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
    • purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP391, available in Jörg Stülke's lab
    • purification from E. coli with N-terminal Strep-tag, in pGP172: pGP823, available in Jörg Stülke's lab
    • purification from E. coli with N-terminal His-tag, in pWH844: pGP1408, available in Jörg Stülke's lab
  • lacZ fusion:
  • translational lacZ fusion (in pAC7): pGP831, available in Jörg Stülke's lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Vladimir Bidnenko, Lei Shi, Ahasanul Kobir, Magali Ventroux, Nathalie Pigeonneau, Céline Henry, Alain Trubuil, Marie-Françoise Noirot-Gros, Ivan Mijakovic
Bacillus subtilis serine/threonine protein kinase YabT is involved in spore development via phosphorylation of a bacterial recombinase.
Mol Microbiol: 2013, 88(5);921-35
[PubMed:23634894] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)