bofA

168

inhibition of the pro-Sigma-K processing machinery

Locus: BSU_00230

Molecular weight: 8.87 kDa

Isoelectric point: 9.32

Protein length: 87 aa

Gene length: 264 bp

Function: control of Sigma-K activation

Product: inhibitor of pro-Sigma-K processing

Essential: no

Synonyms: bofA

Genomic Context

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List of homologs in different organisms, belongs to COG5848 (Galperin et al., 2021)

Gene

Coordinates: 29,772 → 30,035

The protein

Structure

• P24282 (AlphaFold)

Localization

• integral mother cell membrane protein PubMed

Expression and Regulation

Operons

• Genes: dnaX-yaaK-recR-yaaL-bofA

  Description: PubMed

• Genes: bofA

  Description: PubMed

  Regulation

  • expressed during sporulation PubMed

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• MGNA-A081 (bofA::erm), available at the NBRP B. subtilis, Japan
• 1S100 ( bofA::cat), PubMed, available at BGSC
• 1S96 ( bofA::erm), PubMed, available at BGSC
• BKE00230 (ΔbofA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAATCTTCTCATCTCCT,  downstream forward: _UP4_TAATTGATATTATGTATTGA
• BKK00230 (ΔbofA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAATCTTCTCATCTCCT,  downstream forward: _UP4_TAATTGATATTATGTATTGA

Labs working on this gene/protein

• Simon Cutting, London, UK homepage

References

Reviews

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Original Publications

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