yaaH

168

general stress protein, survival of ethanol stress, SafA-dependent protein of the inner spore coat, spore cortex lytic protein, similar to cell wall hydrolase (amides)

Locus: BSU_00160

Molecular weight: 48.47 kDa

Isoelectric point: 5.62

Protein length: 427 aa

Gene length: 1284 bp

Function: survival of ethanol stress, protection of the spore

Product: N-acetylglucosaminidase

Essential: no

Synonyms: yaaH, sleL

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG3858 (Galperin et al., 2021)

Gene

Coordinates: 23,868  25,151

The protein

Protein family

• Glycosyl hydrolase 18 family (according to UniProt)
• Chitinase class II subfamily (with YdhD, according to UniProt)

Domains

• contains two N-acetylglucosamine-polymer-binding LysM domains at the N-terminus (aa 2-46, aa 51-95) PubMed
• glycoside hydrolase family 18 domain (aa 125 to 410)
• LysM domain 1 (aa 2-46) (according to UniProt)
• LysM domain 2 (aa 51-95) (according to UniProt)

Structure

• 4S3K (PDB) (SleL from B. megaterium, 55% identity) PubMed
• P37531 (AlphaFold)

Paralogous protein(s)

• YdhD

Localization

• inner spore coat, localization depends on SafA PubMed

Additional information

• required for survival of ethanol stress PubMed

Expression and Regulation

Operons

• Genes: yaaH

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigE, SpoIIID) PubMed

  Expression

  • expression is heterogeneous PubMed

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon
  • SigB: sigma factor, PubMed, in sigB regulon

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

• Genes: yaaI-yaaH

  Description: PubMed

  Regulation

  • induced by stress (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• MGNA-B889 (yaaH::erm), available at the NBRP B. subtilis, Japan
• BKE00160 (yaaH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATAAATTTGAATGAAAA,  downstream forward: _UP4_TAATTTGGAAACGTCTTTTT
• BKK00160 (yaaH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATAAATTTGAATGAAAA,  downstream forward: _UP4_TAATTTGGAAACGTCTTTTT

References

Reviews

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Original Publications

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