Difference between revisions of "RplB"
| Line 1: | Line 1: | ||
| − | * '''Description:''' [[ribosomal protein]] | + | * '''Description:''' [[ribosomal protein]] L2, required for assembly of [[RplP]] into the 50S subunit of the [[ribosome]]<br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 56: | Line 56: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | + | * essential {{PubMed|23002217,12682299}} | |
| − | essential {{PubMed|23002217,12682299}} | + | * a H142L mutation results in a temperature-sensitive phenotype and is acompanied by growth and [[sporulation]] defects, the mutation can be suppressed by increased expression of ''[[yaaA]]'' {{PubMed|24637032}} |
=== Database entries === | === Database entries === | ||
| Line 84: | Line 84: | ||
* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| + | ** [[RplB]]-[[23S rRNA]] {{PubMed|24637032}} | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
| Line 142: | Line 143: | ||
=References= | =References= | ||
| − | <pubmed> 19653700 9371452 11948165 8635744 10075918 23249812 23002217</pubmed> | + | <pubmed> 19653700 9371452 11948165 8635744 10075918 23249812 23002217 24637032</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 12:05, 19 March 2014
- Description: ribosomal protein L2, required for assembly of RplP into the 50S subunit of the ribosome
| Gene name | rplB |
| Synonyms | |
| Essential | yes PubMed |
| Product | ribosomal protein L2 (BL2) |
| Function | translation |
| Gene expression levels in SubtiExpress: rplB | |
| Interactions involving this protein in SubtInteract: RplB | |
| MW, pI | 30 kDa, 11.013 |
| Gene length, protein length | 831 bp, 277 aa |
| Immediate neighbours | rplW, rpsS |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
translation, essential genes, universally conserved proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU01190
Phenotypes of a mutant
- essential PubMed
- a H142L mutation results in a temperature-sensitive phenotype and is acompanied by growth and sporulation defects, the mutation can be suppressed by increased expression of yaaA PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: ribosomal protein L2P family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- Structure: 1RL2 (L2 RNA-binding domain, Geobacillus stearothermophilus)
- UniProt: P42919
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon: rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Shota Suzuki, Osamu Tanigawa, Genki Akanuma, Hideaki Nanamiya, Fujio Kawamura, Kazumi Tagami, Naofumi Nomura, Teppei Kawabata, Yasuhiko Sekine
Enhanced expression of Bacillus subtilis yaaA can restore both the growth and the sporulation defects caused by mutation of rplB, encoding ribosomal protein L2.
Microbiology (Reading): 2014, 160(Pt 6);1040-1053
[PubMed:24637032]
[WorldCat.org]
[DOI]
(I p)
Josephine R Chandler, Thao T Truong, Patricia M Silva, Mohammad R Seyedsayamdost, Gavin Carr, Matthew Radey, Michael A Jacobs, Elizabeth H Sims, Jon Clardy, E Peter Greenberg
Bactobolin resistance is conferred by mutations in the L2 ribosomal protein.
mBio: 2012, 3(6);
[PubMed:23249812]
[WorldCat.org]
[DOI]
(I e)
Genki Akanuma, Hideaki Nanamiya, Yousuke Natori, Koichi Yano, Shota Suzuki, Shuya Omata, Morio Ishizuka, Yasuhiko Sekine, Fujio Kawamura
Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation.
J Bacteriol: 2012, 194(22);6282-91
[PubMed:23002217]
[WorldCat.org]
[DOI]
(I p)
Matthew A Lauber, William E Running, James P Reilly
B. subtilis ribosomal proteins: structural homology and post-translational modifications.
J Proteome Res: 2009, 8(9);4193-206
[PubMed:19653700]
[WorldCat.org]
[DOI]
(P p)
Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165]
[WorldCat.org]
[DOI]
(P p)
A Nakagawa, T Nakashima, M Taniguchi, H Hosaka, M Kimura, I Tanaka
The three-dimensional structure of the RNA-binding domain of ribosomal protein L2; a protein at the peptidyl transferase center of the ribosome.
EMBO J: 1999, 18(6);1459-67
[PubMed:10075918]
[WorldCat.org]
[DOI]
(P p)
X Li, L Lindahl, Y Sha, J M Zengel
Analysis of the Bacillus subtilis S10 ribosomal protein gene cluster identifies two promoters that may be responsible for transcription of the entire 15-kilobase S10-spc-alpha cluster.
J Bacteriol: 1997, 179(22);7046-54
[PubMed:9371452]
[WorldCat.org]
[DOI]
(P p)
J W Suh, S A Boylan, S H Oh, C W Price
Genetic and transcriptional organization of the Bacillus subtilis spc-alpha region.
Gene: 1996, 169(1);17-23
[PubMed:8635744]
[WorldCat.org]
[DOI]
(P p)
