Difference between revisions of "GabT"
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|style="background:#ABCDEF;" align="center"| '''Product''' || gamma-aminobutyrate transaminase | |style="background:#ABCDEF;" align="center"| '''Product''' || gamma-aminobutyrate transaminase | ||
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || utilization of gamma-amino butyric acid | + | |style="background:#ABCDEF;" align="center"|'''Function''' || utilization of gamma-amino butyric acid |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03900 gabT] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03900 gabT] | ||
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<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
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<br/><br/> | <br/><br/> | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[utilization of amino acids | + | {{SubtiWiki category|[[utilization of amino acids]]}} |
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= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
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** growth defect in the presence of gamma-aminobutyrate {{PubMed|24127574}} | ** growth defect in the presence of gamma-aminobutyrate {{PubMed|24127574}} | ||
| + | ** no growth with gamma-aminobutyrate as single carbon source {{PubMed|24529384}} | ||
=== Database entries === | === Database entries === | ||
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=== Additional information=== | === Additional information=== | ||
| − | + | * the protein was reported to be involved in norspermidine production and biofilm disassembly {{PubMed|22541437}}; however, this is not the case {{PubMed|24529384}} | |
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=The protein= | =The protein= | ||
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* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
** 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate (according to Swiss-Prot) | ** 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate (according to Swiss-Prot) | ||
| − | + | ** the protein was reported to be involved in norspermidine production and biofilm disassembly {{PubMed|22541437}}; however, this is not the case {{PubMed|24529384}} | |
* '''Protein family:''' class-III pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot) | * '''Protein family:''' class-III pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot) | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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=References= | =References= | ||
| − | <pubmed>15223311,12123465,22541437, </pubmed> | + | <pubmed>15223311,12123465,22541437, 24529384 4590473 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 18:16, 28 February 2014
- Description: gamma-aminobutyrate transaminase, general stress protein
| Gene name | gabT |
| Synonyms | ycnG |
| Essential | no |
| Product | gamma-aminobutyrate transaminase |
| Function | utilization of gamma-amino butyric acid |
| Gene expression levels in SubtiExpress: gabT | |
| MW, pI | 47 kDa, 5.304 |
| Gene length, protein length | 1308 bp, 436 aa |
| Immediate neighbours | gabR, gabD |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU03900
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
- the protein was reported to be involved in norspermidine production and biofilm disassembly PubMed; however, this is not the case PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: class-III pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P94427
- KEGG entry: [3]
- E.C. number: 2.6.1.19
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
Your additional remarks
References
Laura Hobley, Sok Ho Kim, Yukari Maezato, Susan Wyllie, Alan H Fairlamb, Nicola R Stanley-Wall, Anthony J Michael
Norspermidine is not a self-produced trigger for biofilm disassembly.
Cell: 2014, 156(4);844-54
[PubMed:24529384]
[WorldCat.org]
[DOI]
(I p)
Ilana Kolodkin-Gal, Shugeng Cao, Liraz Chai, Thomas Böttcher, Roberto Kolter, Jon Clardy, Richard Losick
A self-produced trigger for biofilm disassembly that targets exopolysaccharide.
Cell: 2012, 149(3);684-92
[PubMed:22541437]
[WorldCat.org]
[DOI]
(I p)
Boris R Belitsky
Bacillus subtilis GabR, a protein with DNA-binding and aminotransferase domains, is a PLP-dependent transcriptional regulator.
J Mol Biol: 2004, 340(4);655-64
[PubMed:15223311]
[WorldCat.org]
[DOI]
(P p)
Boris R Belitsky, Abraham L Sonenshein
GabR, a member of a novel protein family, regulates the utilization of gamma-aminobutyrate in Bacillus subtilis.
Mol Microbiol: 2002, 45(2);569-83
[PubMed:12123465]
[WorldCat.org]
[DOI]
(P p)
S Dover, Y S Halpern
Genetic analysis of the gamma-aminobutyrate utilization pathway in Escherichia coli K-12.
J Bacteriol: 1974, 117(2);494-501
[PubMed:4590473]
[WorldCat.org]
[DOI]
(P p)
