MutS

• Description: DNA mismatch repair (mismatch recognition protein)
  
  

Gene name	mutS
Synonyms
Essential	no
Product	mismatch recognition protein
Function	DNA repair
Gene expression levels in SubtiExpress: mutS
Interactions involving this protein in SubtInteract: MutS
MW, pI	97 kDa, 5.189
Gene length, protein length	2574 bp, 858 aa
Immediate neighbours	cotE, mutL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

The gene

Basic information

• Locus tag: BSU17040

Phenotypes of a mutant

• 60-fold increased mutation rate PubMed
• a rnhB mutS-mutL mutant is not viable PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • MutS recognizes mismatches in the DNA and recruits MutL to the site of mismatch recognition

• Protein family: DNA mismatch repair mutS family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • forms dimers PubMed
  • MutS-DnaN PubMed
  • DnaE-MutS PubMed
  • MutL-MutS PubMed

• Localization:
  • forms foci at midcell position, the frequency of foci increases upon mismatch formation PubMed
  • replication-active region of the DNA, via DnaN PubMed

Database entries

• Structure:
  • 1NG9 (from E. coli, 39% identity) PubMed

• UniProt: P49849

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mutS-mutL PubMed

• Expression browser: mutS PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • An antisense RNA is predicted for mutS PubMed
  • intracellular concentration: about 80 dimers (100 nM) PubMed

Biological materials

• Mutant:
  • GP1190 (del mutS-mutL::aphA3) PubMed, available in Jörg Stülke's lab
  • 1A833 ( mutS::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Motohiro Akashi, Hirofumi Yoshikawa
Relevance of GC content to the conservation of DNA polymerase III/mismatch repair system in Gram-positive bacteria.
Front Microbiol: 2013, 4;266
[PubMed:24062730] [WorldCat.org] [DOI] (P e)

Justin S Lenhart, Monica C Pillon, Alba Guarné, Lyle A Simmons
Trapping and visualizing intermediate steps in the mismatch repair pathway in vivo.
Mol Microbiol: 2013, 90(4);680-98
[PubMed:23998896] [WorldCat.org] [DOI] (I p)

Nina Y Yao, Jeremy W Schroeder, Olga Yurieva, Lyle A Simmons, Mike E O'Donnell
Cost of rNTP/dNTP pool imbalance at the replication fork.
Proc Natl Acad Sci U S A: 2013, 110(32);12942-7
[PubMed:23882084] [WorldCat.org] [DOI] (I p)

Justin S Lenhart, Anushi Sharma, Manju M Hingorani, Lyle A Simmons
DnaN clamp zones provide a platform for spatiotemporal coupling of mismatch detection to DNA replication.
Mol Microbiol: 2013, 87(3);553-68
[PubMed:23228104] [WorldCat.org] [DOI] (I p)

Andrew D Klocko, Jeremy W Schroeder, Brian W Walsh, Justin S Lenhart, Margery L Evans, Lyle A Simmons
Mismatch repair causes the dynamic release of an essential DNA polymerase from the replication fork.
Mol Microbiol: 2011, 82(3);648-63
[PubMed:21958350] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Nicole M Dupes, Brian W Walsh, Andrew D Klocko, Justin S Lenhart, Heather L Peterson, David A Gessert, Cassie E Pavlick, Lyle A Simmons
Mutations in the Bacillus subtilis beta clamp that separate its roles in DNA replication from mismatch repair.
J Bacteriol: 2010, 192(13);3452-63
[PubMed:20453097] [WorldCat.org] [DOI] (I p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Mario Pedraza-Reyes, Ronald E Yasbin
Contribution of the mismatch DNA repair system to the generation of stationary-phase-induced mutants of Bacillus subtilis.
J Bacteriol: 2004, 186(19);6485-91
[PubMed:15375129] [WorldCat.org] [DOI] (P p)

Meindert H Lamers, Herrie H K Winterwerp, Titia K Sixma
The alternating ATPase domains of MutS control DNA mismatch repair.
EMBO J: 2003, 22(3);746-56
[PubMed:12554674] [WorldCat.org] [DOI] (P p)

F Ginetti, M Perego, A M Albertini, A Galizzi
Bacillus subtilis mutS mutL operon: identification, nucleotide sequence and mutagenesis.
Microbiology (Reading): 1996, 142 ( Pt 8);2021-9
[PubMed:8760914] [WorldCat.org] [DOI] (P p)